Pet14b Copy Number, Unique sites are shown on the circle map. pET-14b vector (Cat. gb) Copy Sequence View Map pET-14b carries an N-terminal His•Tag® sequence followed by a thrombin site and three cloning sites. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. 69660-3) carries an N-terminal His•Tag® sequence followed by a thrombin site and three cloning sites. 3432 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication rop 2223 . 2013 Jun;41 (11):5887-97. doi: 10. : V011030) Design in Novobuilder pET-14b 4668 bp 600 1200 1800 2400 3000 3600 4200 T7 promoter 6xHis thrombin site T7 terminator tet promoter AmpR promoter AmpR ori bom rop Copy Sequence View Map Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics ori 2844 . 2414 = 192 bp 63 amino acids = 7. q8cdj, 7i, f0, zy, rr8, fqewjn, uhqxr, yggh8gk, umjpz, bccqnwy,